Sperm phenotype and its relationship to somatic and germ line genotype: a study using mouse aggregation chimeras.
نویسنده
چکیده
The mouse strains C3H/Bi McL and C57BL/McL were shown to have markedly different spermatozoa, and, by combining four different sperm dimensions by means of a discriminant function, it was possible to identify individual spermatozoa from the two strains with a calculated misclassification probability of 2.1%. Hybrids had intermediate sperm discriminant values. The sperm from five C3H++ C57 chimeras were characterized using the discriminant function, and it was found that one chimera had C57-like sperm, another had C3H-like sperm, while each of the other three had sperm of both phenotypes. In no case did the sperm populations of chimeras resemble those of hybrids. A detailed analysis of the sperm dimensions of the chimeras showed that the differences between the two populations of sperm and the variances of these populations are the same as for C3H and C57 sperm populations from pure strain mice. These observations imply that sperm dimensions are determined at the level of individual spermatozoa but do not differentiate between intrinsic (germ line) and extrinsic (Sertoli cell) control. However, the proportions of C3H-type and C57-type sperm in the chimeras were found to be correlated with the proportions of C3H-derived and C57-derived offspring but not with the proportions of C3H and C57 cells in somatic tissues. It is argued that the differences in sperm dimensions between the two strains are due to genes expressed through the germ line.
منابع مشابه
I-18: Avian Chimeras and Germ Cell Migration
Background: In avian species, the germ line stem cell population arises outside of the embryonic gonad and proceeds on a circuitous migration to the germinal epithelium. Specifically, in the avian embryo, the process of germ line stem cell migration proceeds through a series of active and passive migratory phases. The germline stem cells or primordial germ cells (PGCs) located in the epiblast o...
متن کاملI-37: Genome Instability and DNA Damage in Male Somatic and Germ Cells Expressed as Chromosomal Microdeletion and Aneuploidy Is A Major Cause of Male Infertility
Background: Sperm chromatin insufficiencies leading to low sperm count and quality, infertility and transmission of chromosomal microdeletion and aneuploidies to next generations can be due to exposure to environmental pollutions, chemicals and natural or manmade ionizing radiation. In this project which has continued for more than 10 years and is unique in many technical aspects in Iran and in...
متن کاملGeneration of Hprt-disrupted rat through mouse←rat ES chimeras.
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse←rat ES chimeras....
متن کاملP-48: Nicotine Alters Both Somatic and Germ Cells in Adult Mouse Testis
Background: Nicotine as a toxic agent in cigarette has detrimental effects on reproduction. The aim of this study was to evaluate effects of of nicotine on germ and somatic cells in adult mouse testis. Materials and Methods: Male mice were divided into four groups. Group A or controls, groups of B, C and D were treated with nicotine intraperitoneally in doses of 0.1, 0.2 and 0.4 mg/100 g body w...
متن کاملI-13 Infertility with Impaired Zona Pellucida Adhesion of Spermatozoa from Mice LackingTauCstF-64
Background: Fertilization is a multistep process requiring spermatozoa with unique cellular structures and numerous germ cell-specific molecules that function in the various steps. In the highly coordinated process of male germ cell development, RNA splicing and polyadenylation help regulate gene expression to ensure formation of functional spermatozoa. Male germ cells express tauCstF-64 (Cstf2...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Developmental biology
دوره 44 1 شماره
صفحات -
تاریخ انتشار 1975